Translational Genomics (NGS)
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Equipment
Laboratory 1 - Preamplification Zone
- Chemagic Magnetic Separation Module I (Chemagic MSM I) (Chemagen, PerkinElmer)
- Nanodrop 2000 spectrophotometer
- Agilent 2200 TapeStation System bioanalyzer
- Qubit 2.0 fluorometer
- Thermoscientific Varioskan LUX
- Freezers -20 ºC
Laboratory 2 - Post-Amplification Zone
- 2 System 9700 PCRs from AppliedBiosystems
- VeritiPro 96-Well ThermalCycler from AppliedBiosystems
- MiSeq sequencer from Illumina
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Noteworthy
- Members of the Global Alliance for Genetics & Health (GA4GH) since 2014
- Cooperation agreements signed with Sistemas Genómicos and Parque Científico de Madrid.
- Teachers of the course on Translational Research Techniques.
- Collaboration agreements signed with several technology companies belonging to ASEBIO (Spanish Association of Biocompanies), and with national and international public and private universities.
- Lecturers in courses on Translational Research Techniques, Omic Sciences and Massive Sequencing Techniques (NGS).
- Quality assurance in the storage and processing of samples, maintaining their traceability, following standardised work protocols (SOPs). Laboratory and equipment cleaning, maintenance and verification plan.
- Continuous training of laboratory staff.
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Service portfolio and prices
**Rates are available for all services. Please, consult at the Unit
Nucleic acid extraction - DNA*Service Unit Conditions DNA extraction from blood 2 ml_manual €/sample or reaction 1 sample DNA extraction from 250 mg faeces_manual 1 sample DNA extraction from blood 5 ml_manual_head 12 1 sample DNA extraction from blood 400 µl_head 96 96 samples DNA extraction from 2 ml saliva_head 12 Consult price for < 12 samples (1) DNA extraction from dried blood (paper 3.2 mm)_head 96 Consult price for < 96 samples (1) DNA extraction from fresh tissue 10 mg (tissue or animal cells)_head 12 Consult price for < 12 samples (1) DNA extraction from fresh tissue 10 mg (tissue or animal cells)_head 96 DNA extraction from FFPE tissue 10 µm section_head 96 Consult price for < 96 samples (1) DNA extraction from 1,5 ml bacterial culture_head 96 Consult price for < 96 samples (1) RNA extraction
Service Unite Conditions RNA extraction from 2,5 ml of blood in PAX-gene tubes_head 12 €/sample or reaction Consult (1) Special DNA and RNA extraction Ask for other fees: DNA from amniotic fluid, plasma, buccal swab, viral DNA/RNA, etc. Consult (1) Nucleic acid analysis (1)
Service Unit Conditions High sensitivity DNA analysis by capillary electrophoresis_Tape Station 2200 €/sample or reaction No. of samples of 48 or more units consult price list (1) Wide range DNA analysis by capillary electrophoresis_Tape Station 2200 Genomic DNA analysis by capillary electrophoresis_Tape Station 2200 High sensitivity RNA analysis by capillary electrophoresis_Tape Station 2200 Wide range RNA analysis by capillary electrophoresis_Tape Station 2200
Nucleic acid quantificationService Unit Conditions DNA or RNA quantification by UV absorbance (Nanodrop) and by fluorimetry (Qubit 2.0) €/sample or reaction Quantification of DNA or RNA by UV absorbance (Nanodrop) Quantification of DNA or RNA by UV absorbance (Nanodrop). 96-sample plate. Minimum no. of samples = 48 Quantification of DNA or RNA by UV absorbance (Nanodrop). Self-service (6) Quantification of DNA or RNA by fluorimetry_Qubit 2,0 Quantification of DNA or RNA by fluorimetry_Qubit 2,0. 96-sample plate. Minimum no. of samples = 48 Quantification of DNA or RNA by fluorimetry_Qubit 2.0. Self-service (6)
Primary bioinformatics analysis of sequencing data Molecular determination in biological fluids
Sanger sequencingService Unit Sanger sequencing tube €/sample or reaction Sanger sequencing plaque (48/96)
NGS library constructionService Library construction (2) Metagenomics (RNAr 16S sequencing)
NGS library construction + MiSeq run (5)Service Run Kit v2 PE: 2X150pb (300 cicles): ~30 million reads Run Kit v3 PE: 2X75pb (150 cicles): ~50 million reads Run Kit v3 PE: 2X300pb (600 cicles): ~50 million reads Run Kit v2 Micro PE: 2X150pb (300 cicles): ~8 million reads Run Kit v2 Micro PE: 2X150pb (300 cicles): ~2 million reads Run Kit v2 Micro PE: 2X150pb (500 cicles): ~2 million reads Run in MiSeq (includes cartridge and run reagents) (3) (5) Run Kit v2 PE: 2X150pb (300 cicles): ~30 million reads Run Kit v3 PE: 2X75pb (150 cicles): ~50 million reads Run Kit v3 PE: 2X300pb (600 cicles): ~50 million reads Run Kit v2 Micro PE: 2X150pb (300 cicles): ~8 million reads Run Kit v2 Nano PE: 2X150pb (300 cicles): ~2 million reads Run Kit v2 Nano PE: 2X250pb (500 cicles): ~2 million reads
MiSeq run (equipment use only) (4)Service Run 150 cicles Run 300 cicles Run 600 cicles
NGS library construction + NextSeq 550 run (5)Service Run Kit v2 NextSeq High Output PE: 2X150pb (3000 cicles): >400 million reads Run Kit v2 NextSeq High Output PE: 2X75pb (150 cicles): >400 million reads Run Kit v2 NextSeq Mid output PE: 2X150pb (3000 cicles): >130 million reads Run Kit v2 NextSeq Mid output PE: 2X75pb (150 cicles): >130 million reads
NextSeq 550 run (includes cartridge and run reagents) (3) (5)Service Run Kit v2 NextSeq High Output PE: 2X150pb (3000 cicles): >400 million reads Run Kit v2 NextSeq High Output PE: 2X75pb (150 cicles): >400 million reads Run Kit v2 NextSeq Mid output PE: 2X150pb (3000 cicles): >130 million reads Run Kit v2 NextSeq Mid output PE: 2X75pb (150 cicles): >130 million reads
NextSeq 550 (equipment use only) (4)150 cicles run 300 cicles run
NovaSeq 6000 run (1)Service Runs from 800 million reads
CGH arrays (comparative genome hybridisation)Service CGH 60k array CGH 180k-HD array CGH 180k-SNP array
MLPAService Unit Conditions MLPA €/sample or reaction For several samples, please consult prices (1)
Microsatellite genotypingService Microsatellite analysis
CitogeneticsService Unit Karyotype €/sample or reaction FISH
Miscellaneous (1)Service Genomic DNA controls Real-time PCR/Allelic discrimination Other genetic/genomic determinations/studies
ServiceService Unit Use of thermocyclers €/hour PCR product purification using ExoSap €/sample or reaction
ConsultancyService Unit Design of experiments/oligonucleotides €/hour Experimental technical advice €/hour
Bioinformatic analysis (performed by the Bioinformatics Unit) (1)Service Secondary and/or tertiary analysis of raw data NOTES
- Please consult rates, as the basic cost varies for each application and for each experiment.
- Re-sequencing of human gene panels (PCRs user, multiplex PCRs, capture), RNAseq, mitochondrial genome, microorganism genomes, influenza A virus, 16S metagenome, aptamers, miRNAs, etc.
- Running reagents + flowcell + use of equipment.
- Use of equipment only; the user provides the cartridge and reagents for the run.
- According to budget.
- Self-service includes use of equipment always supervised by Unit staff.