• Three qRT-PCR kits (Lightcycler 480).
• Conventional thermal cycler (2720 Thermal Cycler, Applied Biosystems).
• Equipment for in situ hybridisation (DAKO).
• Nucleic acid quantification equipment: Bioanalyzer, NanoDrop, Qubit 4 Fluorometer.
• Microtome and paraffin block bath.
• Spectophotometer (Multiskan Go, Thermo.
• Thermoblock (ThermoMixer F1.5, Eppendorf).
• Inverted fluorescence microscope with acloped camera and computer.
• Cell Culture Unit: P2 laminar flow hoods, regular CO2 incubators, hypoxia incubator, thermostatised bath, inverted light microscope, electroporator and nitrogen tank.

• They have developed a method for the diagnosis and prognosis of acute renal failure protected by two patent families (P200901825 and P2011320232), both with national phase entry in eight countries.
• They are developing the use of miR-127 as a new therapeutic agent in renal fibrosis, protected by patent EP 16 382 399.0, granted in the US and Europe.
• They are developing the use of miR-326 as a new therapeutic agent in cancer, protected by patent EP22383047.2.
• National Projects: 
  • DTS22/00101 "Multiplatform for precision medicine in rectal cancer (MIRaMut).
  • Public-Private collaboration project of the Ministry of Science and Innovation, PanCaTEST: "Development and clinical validation of a genetic signature for non-invasive diagnosis of pancreatic cancer and precursor lesions".
• International projects:
  • EATRIS-Plus project (H2020-INFRADEV-2019-2, 4. 9 M€) dedicated to the identification and validation of biomarkers for clinical use, particularly miRNAs.
  • Project "REMEDI4ALL: Building a Sustainable European innovation platform to enhance the repurposing of medicines for all". (HORIZON-HLTH-2021-DISEASE-04), 25 M€, dedicated to drug repurposing based on reliable biomarkers.
• Clearly translational services with expertise in renal pathology, renal preservation, oncology, sepsis, cardiology, etc.

**Consult prices with the unit

miRNAs.- Sample preparation

RNA extraction and quantification. Total RNA extraction enriched in small RNAs (tissue, paraffin tissue *, cells, serum, plasma, other biofluids)
* May have associated costs of paraffin tissue sections

• NanoDrop RNA analysis and quantification
• Analysis and quantification of RNA Biochip

miRNAs: Determination by qRT-PCR (LightCycler 480 SYBR green)

RNA reverse transcription; RNA Spike-in kit, UniRT; Real-time PCR (by miRNA and by triplicate sample); Data analysis with the appropriate software.

Array of miRNAs: sample preparation

RNA extraction and quantification. Total RNA extraction enriched in small RNAs (tissue, paraffin tissue *, cells, serum, plasma, other biofluids)
* May have associated costs of paraffin tissue sections

NanoDrop RNA analysis and quantification
Analysis and quantification of RNA Biochip

Array of miRNAs: retrotranscription and array

RNA retrotranscription (in 3 volumes); RNA Spike-in kit, UniRT; Syber; Array 1 panel (conf.A + Conf. B, 762 miRNAs); Data analysis using the appropriate software.

Modulation of miRNAs in vitro

Cell culture and transfection

Location of miRNAs by in situ hybridization

* May have associated costs of paraffin tissue sections

Histological section of paraffin blocks