Biomarkers and therapeutic targets

Leader

Elisa Conde Moreno

Personnel

Edurne Ramos Muñoz (técnico apoyo)

Apoyo del grupo de Biomarcadores y Dianas Terapéuticas del IRYCIS, liderado por la Dra. Laura García Bemerjo

  • Equipment
    • Two devices for qRT-PCR (Lightcycler 480)
    • Equipment for in situ hybridization (DAKO)
    • Equipment for the quantification of nucleic acids: Bioanalyzer, NanoDrop
    • Microtome and bath for paraffin blocks
    • Spectrophotometer
    • Inverted fluorescence microscope with camera and computer
    • Cell Culture Unit: P2 laminar flow hoods, regular CO2 incubators, hypoxia incubator, RCTA device for cellular impedance, thermostated bath, inverted light microscope, electroporator and nitrogen tank.
  • Noteworthy
    • We have developed a diagnostic and prognostic method for acute renal failure protected by two patent families  (P200901825  and  P2011320232), both with entry into national phases in eight countries.
    • Clear translational services with experience in renal pathology, renal preservation, oncology, sepsis, cardiology, etc.
  • Services

    miRNAs.- Sample preparation

    RNA extraction and quantification. Total RNA extraction enriched in small RNAs (tissue, paraffin tissue *, cells, serum, plasma, other biofluids)
    * May have associated costs of paraffin tissue sections

    • NanoDrop RNA analysis and quantification
    • Analysis and quantification of RNA Biochip

    miRNAs: Determination by qRT-PCR (LightCycler 480 SYBR green)

    RNA reverse transcription; RNA Spike-in kit, UniRT; Real-time PCR (by miRNA and by triplicate sample); Data analysis with the appropriate software.


    Array of miRNAs: sample preparation

    RNA extraction and quantification. Total RNA extraction enriched in small RNAs (tissue, paraffin tissue *, cells, serum, plasma, other biofluids)
    * May have associated costs of paraffin tissue sections

    NanoDrop RNA analysis and quantification
    Analysis and quantification of RNA Biochip

    Array of miRNAs: retrotranscription and array

    RNA retrotranscription (in 3 volumes); RNA Spike-in kit, UniRT; Syber; Array 1 panel (conf.A + Conf. B, 762 miRNAs); Data analysis using the appropriate software.


    Modulation of miRNAs in vitro

    Cell culture and transfection


    Location of miRNAs by in situ hybridization

    * May have associated costs of paraffin tissue sections


    Histological section of paraffin blocks