Microarrays

Leader

Javier Martínez Botas

Contact

+34 91 336 84 66

  • Equipment
    • Microarray printing robot (SpotArray 72, PerkinElmer)
    • Reading scanner (ScaArray Express, PerkinElmer)
    • Liquid handling robot (Janus Varispan, PerkinElmer)
    • Semiautomatic hybridizer (BioMixer ™ II, CapitalBio)
    • Real-time thermal cycler (LightCycler 480, Roche)
    • Computer support for microarray analysis
    • Three freezers connected to a radio control alarm system
  • Highlights

    Personalised microarray design

  • Service portfolio

    Preparation of samples

    • Total RNA extraction (Tripure) - 1 sample
    • Total RNA extraction (Columns) - 1 sample
    • mRNA extraction (Columns) - 1 sample
    • RNA analysis and quantification (NanoDrop and integrity gel) - 8 samples

    Microarrays

    • DNA and protein microarray printing *
    • Retrotranscription and RNA labeling (two-color labeling with Cy3 or Cy5 without amplification)
    • Hybridization (DNA or proteins) **
    • Microarray reading (DNA or proteins)

    Real-time PCR (LightCycler 480 SYBR green)

    • Probe design (for SYBR green)
    • RNA retranscription
    • Real-time PCR (by gene and by triplicate sample)

    * Price of printing or crystals is not included

    ** Price of the microarray is not included