Molecular quantification and characterisation

Leader

Oscar Pastor Rojo

Contact

opastor.hrc(ELIMINAR)@salud.madrid.org

Bioquímica Investigación. Planta - 1 dcha

  • Equipment
    • High quantitative resolution LC-MS system:
      • Refrigerated injector
      • ESI and APCI sources
    • GC-MS system
    • LC-ELSD system
    • Three HPLC devices with array diode, fluorescence, visible UV and electrochemical detectors.
    • Gas Chromatography and Mass Spectrometry
  • Services
    • Analysis of fatty acids and sterols.
    • High sensitivity quantification of specific groups of metabolic intermediaries.
    • Lipidomics: analysis of phospholipids, triglycerides, cholesterol esters and other intermediates at the molecular level.
    • Lipid composition analysis at group level (esterified cholesterol, free cholesterol, free fatty acids, phospholipids, sphingolipids)
    • Sugar Analysis
  • Service portfolio

    Lipid base extraction (10 samples)

    Extraction with organic solvents to isolate lipids from the sample

    Sphingolipid Extraction (10 samples)

    Expanded lipid extraction to extract the sphingolipid class


    Standard lipidomics (CE, TG, PE, PC, DG, LPC, LPE, SM) (10 samples)

    Analysis of molecular species belonging to the classes: cholesterol esters (EC), triglycerides (TG), diglycerides (DG), choline phospholipids (LPC, PC, SM) and ethanolamine phospholipids (LPE, PE)


    Sphingolipid lipidomics (SM, Cer, dhCer, HexCer, LacCer, Sulf) (10 samples)

    Analysis of molecular species of sphingolipids belonging to the classes: sphingomyelin (SM), ceramides (Cer, dhCer), hexosylceramides (HexCer), lactosylceramides (LacCer) and Sulfates (Sulf)


    Lipidomic phospholipid ACIDS (PI, PS, CL, PG, BMP) (10 samples)

    Analysis of molecular acid lipid species: phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylglycerols (PG, BMP) and cardiolipins (CL)


    Bioinformatic analysis (10 samples)

    Includes processing and filtering of mass signals, integration of peaks, correction of sample quantity, correction by internal standard, quantitative report issuance, graphs and principal component analysis (PCA).