Lipids and lipoproteins

Leaders

Miguel Ángel Lasunción Ripa

Diego Gómez-Coronado

Contact

Tlf: +34 91 336 80 77 / +34 91 336 86 84

  • Equipment
    • HPLC with UV and radioactivity detectors.
    • Crop cabin and stove.
    • Radioactivity counter
    • Ultracentrifuge
    • Autoanalyzer
    • Electrophoresis chamber
  • Service portfolio

    Sterols analysis

    Analysis of sterols (cholesterol, desmosterol, 7-dehydrocholesterol, etc.) in plasma, tissues or cells. The study includes the extraction of saponifiable lipids and the separation of sterols by HPLC and their quantification using the specific absorbance.

    Estimated execution time of 15 days


    Cholesterol biosynthesis

    Cholesterol biosynthesis (incorporation of radioactive acetate into the different sterols of the biosynthetic pathway). The study includes the incubation of the cells in the presence of the tracer, the extraction of lipids, the separation of sterols by HPLC and the quantification of their radioactivity.

    Estimated execution time of 15 days


    Human low density lipoprotein (LDL) for research

    Human low density lipoprotein (LDL) for research. Isolated by ultracentrifugation and characterized.

    Estimated execution time according to max availability. 20 days


    Acetylated low density lipoprotein (LDL), for research use

    Acetylated low density lipoproteins (LDL) for research use. Human LDL isolated by ultracentrifugation, marked by acetylation and characterized.

    Estimated execution time according to max availability. 20 days


    Low density lipoproteins (LDL) labeled with DiI, for research use

    Low density lipoproteins (LDL) labeled with DiI for research use. Human LDL isolated by ultracentrifugation, labeled with DiI and characterized.

    Estimated execution time according to max availability. 20 days